Over the past 20 years, Eurofins Tecna has gained a prevalent role in the Mediterranean area for its reliable and versatile screening solutions for the aflatoxin M1 in dairy products.
I’screen AFLA M1 milk is the world first ELISA test kit to be granted, in 2020, with the PTM status by AOAC International, for the quantitative analysis of aflatoxin M1 in raw bovine milk, skim milk and milk powder. The study included a third party independent verification that confirmed excellent performances of the assay. The kits is fully compliant to the ISO 14675 guideline – having adequate reagents format and assay layout, conform selectivity, specificity, sensitivity and accuracy.
An alternative version is represented by I’screen AFLA M1, an ELISA test kit built with same reagents and layout of I’screen AFLA M1 milk, that includes the applications and buffers needed for the analysis of several dairy matrices: ewe, goat, buffalo milk, UHT whole and skim milk, yoghurt, sour cream, cheese, firm cheese and mozzarella cheese. Although it is always recommended to prevent cheese contamination with an earlier milk verification, the availability of a kit that is validated for dairy finished product represents an advantage – also for Public Institutes and Official control bodies, being therefore able to run sampling and screening of finished goods.
Both the test kits share the possibility to analyse the milk without running any sample preparation. It is not necessary to centrifuge and defat the samples, milk of different animals can be run in the assays with their natural content of fat without relevant difference to results obtained for skimmed milk. Fat removal through centrifugation is an option, as prescribed by the ISO 14675, leading to excellent results. The analyst can hence run the method of choice always with reliable results.
Both the test kits have been successfully validated on the automatic analyzer The Bolt: a compact, reliable, walk-away instrument that enable a complete analytical shift from the technicians, thus guaranteeing free time for the personnel, a performance standardization and a full automatic analysis of milk.
The I’screen kits include seven standards to be run within each session. A low cost option is represented by the B ZERO AFLA M1, a virtual – master curve calibrated assay. No wells are needed to be spent for the calibration, thus producing a relevant savings for the end-user. The sample quantification is supported by a batch-related calibration provided in the Certificate of Analysis of each kit, that is stable and valid through the whole kit shelflife.
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